Yemen’s Tehran-aligned Houthis have claimed responsibility for an attack on a refinery in Saudi Arabia, two days after the kingdom signed a defence pact with Turkey and Pakistan in response to growing regional instability caused by the US-Israel war on Iran.

The attack early on Sunday came after Iran issued tough new demands to reopen the strait of Hormuz, and the United Arab Emirates said one of its ships was targeted by an Iranian missile, as a deal to open the strategic waterway remained out of reach.

Iran’s supreme national security council said the strait would not open until the US corrected its “behaviour”. The US must never threaten Iran again and permanently end the war with Tehran and its armed allies in the region, said the statement from the council’s secretary, Mohammad Bagher Zolghadr, a former commander in the Islamic Revolutionary Guard Corps.

He added that the US must also end the naval blockade of Iranian ports and withdraw its military from the area, “completely compensate” Iran for war damage, lift sanctions and “unconditionally” release frozen assets.

There was no immediate comment from the US. According to the memorandum of understanding (MoU) signed by the US and Iran in June, a timetable to end sanctions and a plan for compensation would be part of a final deal, and that negotiations would address frozen assets.

The Iranian foreign minister, Abbas Araghchi, said on Saturday that Iran and Oman were close to reaching an agreement on navigation through the strait of Hormuz, “specifically the determination of a transit route”. But the waterway’s reopening was contingent on other conditions, Araghchi said, in remarks shared on social media. He blamed the situation on what he called the US violation of the June MoU.

Oman, which is mediating and has made relatively few comments on the Hormuz talks, said on Saturday that the discussions were continuing “in a positive and constructive atmosphere”, and condemned attacks on ships in the strait.

With Iran and the US still arguing over the parameters for peace, the Houthis last month declared a naval blockade against Saudi Arabia in the Red Sea, citing what they said was a Saudi siege on them, an allegation Riyadh denies.

The Saudi energy ministry said a fire had broken out at Aramco’s Jazan refinery early on Sunday but was later extinguished with no injuries and authorities were dealing with the incident, without giving a cause.

The Houthis used a drone to target the refinery in south-west Saudi Arabia, Yahya Saree, a military spokesperson for the rebel group, said in a post on X. They had struck Aramco sites before in Jazan, which processes 400,000 barrels of crude oil a day. They had also hit Yanbu, an oil export hub on the Red Sea.

In a separate statement, Saree said the Houthis also carried out missile and drone strikes on the Yemeni Red Sea port city of Mocha, which is controlled by the country’s internationally recognised, Saudi-backed governing body in Aden.

Iran and its allied Shia armed groups have been striking Saudi Arabia and other Gulf states and blockading their energy shipments since the US and Israel launch attacks on 28 February.

The escalation of the conflict, which has disrupted global energy supplies and rattled the world’s economy, prompted Saudi Arabia on Friday to form an alliance with US allies Turkey and Pakistan.

The accord is intended to strengthen collective deterrence against any act of aggression and stipulates that an armed attack against any of the three would be regarded as an attack on all.

The Turkish foreign minister, Hakan Fidan, said the alliance was not directed against Iran or any other country, but rather served as a general pledge to support the security of Turkey, Saudi Arabia and Pakistan. It was not clear whether or how either Pakistan or Turkey would join in any Saudi response to the latest attacks.